pRL-TK induction can cause misinterpretation of gene promoter activity.

1.Cain, S.R. and S. Ganguly. 1995. Uses of fusion genes in mammalian transfection, p. 9.6.1-9.6.12. In F.D. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl (Eds.), Current Protocols in Molecular Biology. John Wiley & Sons, New York. 2.Goridis, C. and J.F. Brunet. 1999. Transcriptional control of neurotransmitter phenotype. Curr. Opin. Neurobiol. 9:47-53. 3.Hong, S.J., C.H. Kim, and K.S. Kim. 2001. Structural and functional characterization of the 5′ upstream promoter of the human Phox2a gene: possible direct transactivation by transcription factor Phox2b. J. Neurochem. 79:1225-1236. 4.Howard, M., D.N. Foster, and P. Cserjesi. 1999. Expression of HAND gene products may be sufficient for the differentiation of avian neural crest-derived cells into catecholaminergic neurons in culture. Dev. Biol. 215:62-77. 5.Howard, M.J., M. Stanke, C. Schneider, X. Wu, and H. Rohrer. 2000. The transcription factor dHAND is a downstream effector of BMPs in sympathetic neuron specification. Development 127:4073-4081. 6.Kim, H.S., H. Seo, C. Yang, J.F. Brunet, and K.S. Kim. 1998. Noradrenergic-specific transcription of the dopamine β-hydroxylase gene requires synergy of multiple cis-acting elements including at least two Phox2a-binding sites. J. Neurosci. 18:8247-8260. 7.Luckow, B. and G. Schutz. 1987. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 15:5490. 8.Reissmann, E., U. Ernsberger, P.H. FrancisWest, D. Rueger, P.M. Brickell, and H. Rohrer. 1996. Involvement of bone morphogenetic protein-4 and bone morphogenetic protein-7 in the differentiation of the adrenergic phenotype in developing sympathetic neurons. Development 122:2079-2088. 9.Seo, H., S.J. Hong, S. Guo, H.S. Kim, C.H. Kim, D.Y. Hwang, O. Isacson, A. Rosenthal, and K.S. Kim. 2002. A direct role of the homeodomain proteins Phox2a/2b in noradrenaline neurotransmitter identity determination. J. Neurochem. 80:905-916. 10.Varley, J.E., C.E. McPherson, H. Zou, L. Niswander, and G.D. Maxwell. 1998. Expression of a constitutively active type I BMP receptor using a retroviral vector promotes the development of adrenergic cells in neural crest cultures. Dev. Biol. 196:107-118. 11.Yang, C., H.S. Kim, H. Seo, C.H. Kim, J.F. Brunet, and K.S. Kim. 1998. Paired-like homeodomain proteins, Phox2a and Phox2b, are responsible for noradrenergic cell-specific transcription of the dopamine β-hydroxylase gene. J. Neurochem. 71:1813-1826.